植物生长调节剂对城市污水中小球藻生长和油脂积累的影响

摘要

研究了不同质量浓度植物生长调节剂(IAA、TRIA、6-BA、2,4-D)对蛋白核小球藻(Chlorella pyrenoidosa)生长和油脂积累的影响,并从藻细胞形态,脂肪酸组成及元素含量等方面对机理进行了探讨.结果表明:2,4-D对城市污水中小球藻生长的促进作用最为显著,当2,4-D的质量浓度为1.5 mg/L时小球藻干重和油脂产量分别达到最大值,比空白对照组分别提高了30.77％和51.43％.利用扫描电镜观察可以发现处理组小球藻个体大小略大于空白对照组.通过对生成的脂肪酸甲酯进行气相色谱分析,结果显示植物生长调节剂的投加对小球藻脂肪酸组成影响不大,但提升了单不饱和脂肪酸含量,有利于提高所得生物柴油的品质.%Effects of different mass concentrations of plant growth regulator (IAA、TRIA、6-BA、2,4-D) on growth and oil accumulation of Chlorella pyrenoidosa were studied.The mechanism was also discussed from algal cell morphology,fatty acid composition and element content,etc.The results showed that 2,4-D significantly promoted growth of Chlorella pyrenoidosa in municipal wastewater.The dry weight and lipid yield of Chlorella pyrenoidosa reached the maximum when mass concentration of 2,4-D was 1.5 mg/L,and increased by 30.77％ and 51.43％ compared with control group,respectively.It was found that the size of Chlorella pyrenoidosa in treatment group was slightly larger than that in control group by scanning electron microscope.According to the GC analysis of the lipids,plant growth regulator exerted a relatively low influence on the fatty acid composition of Chlorella pyrenoidosa,but it increased the content of monounsaturated fatty acid,which was helpful to improve the quality of biodiesel.