腹腔镜结直肠癌手术外周血细胞角蛋白20mRNA表达的研究

摘要

目的 研究细胞角蛋白20mRNA(CK20mRNA)在腹腔镜结直肠癌手术患者外周血中的表达情况,探讨腹腔镜治疗结直肠癌手术对肿瘤微转移的影响.方法 将44例结直肠癌患者采用单盲随机方法分为腹腔镜组25例与开腹组19例,采用逆转录聚合酶链反应检测患者术前、术中、术后外周血中CK20mRNA的表达,对132例次标本进行CK20mRNA检测的研究.结果 腹腔镜组CK20mRNA阳性表达25例次,阴性表达50例次;开腹组:CK20mRNA阳性表达22例次,阴性表达35例次;2组间比较无统计学意义.结论 腹腔镜结直肠癌手术患者外周血术前、术后CK20mRNA表达与开腹手术无统计学意义,腹腔镜结直肠癌手术在肿瘤微转移方面是安全、可行、有效的.%Objective To study the possible influence of laparoscopic surgery on tumor micrometastasis via detecting the expression of CK2OmRNA in patients with coloreetal cancer. Methods Forty-four cases of colorectal cancer were divided into two groups: the laparoscopic surgery group(25 cases) and open surgery group( 19 cases).Blood samples were obtained 24 hours before the operation, intra-operation after finishing anastomosis, and 48 hours after operation respectively. RT-PCR was applied to detect the expression of CK20mRNA before-, intra- and post-operation. Results In the laparoscopic surgery group, 25 cases were CK20mRNA positive, whereas 50 cases were negative. The before-, intra-, post-operation expression of CK20mRNA positive cases were 8, 9, 8, respectively.In the open surgery group, 22 cases were CK20mRNA positive, whereas 35 cases were negative. The before-,intra-, post-operation expression of CK20mRNA positive cases were 9, 6, 7, respectively. Conclusion There was no significant difference of the expression of CK20mRNA before- and post-operation between the two groups.Laparoscopic surgery for colorectal cancer had little influence on tumor micremetastasis.