Endoglin与血管内皮生长因子和血管内皮生长因子受体2及基质金属蛋白酶9联合表达与宫颈癌血管生成的关系

摘要

Objective To observe the co-expression of endoglin, vascular endothelial growth factor (VEGF), kinase insert domain containing receptor (KDR), and matrix metalloproteinase-9 (MMP-9) and their role of regulating regional tumor angiogenesis in invasive carcinoma of cervix. Method The expression of endoglin, VEGF, KDR and MMP-9 and the microvessel density (MVD) labeled by CD34 in 15 cases of normal cervical epi-thelium (NCE), 18 cases of cervical intraepithelial neoplasm (CIN) and 75 cases of invasive carcinoma of cervix (ICC) were detected by SP immunohistochemistry technique. Results The positive rote of endoglin, VEGF, KDR and MMP-9 was 6.7%, 26.7%, 13.3% and 20.0% in NCE group; 38.9%, 61.1%, 61.1% and 55.6% in CIN group; 64.0%, 77.3% , 69.3% and 81.3% in ICC group. The positive rate of endoglin, VEGF, KDR and MMP-9 all increased remarkably in 3 groups (P < 0.05). In ICC group, the MVD was positively related to the ex-pression of endoglin(r = 0.499, P < 0.01), VEGF (r = 0.602, P < 0.01), KDR (r = 0.331, P < 0.01) and MMP-9 (r = 0.287, P < 0.05), respectively. In the cases with all positive expression of endoglin, VEGF, KDR and MMP-9 in ICC group, the MVD was significantly higher than those without positive expression (P < 0.01). Conclusion Co-expression of endoglin, VEGF, KDR and MMP-9 may play a key role in up-regulating regional tumor angiogenesis in cervical carcinoma. Co-overexpression of all endoglin, VEGF, KDR and MMP-9 in cervical carcinoma may result in rapid tumor tmgiogenesis.%目的 探讨Endoglin、血管内皮生长因子(VEGF)、血管内皮生长因子受体2(KDR)及基质金属蛋白酶9(MMP-9)联合表达在宫颈癌局部血管生成中的作用.方法 采用免疫组织化学SP法检测15例正常宫颈上皮(NCE)、18例宫颈上皮内瘤变(CIN)和75例宫颈癌(ICC)组织Endoglin、VEGF、KDR及MMP-9表达,并检测CD34标记的微血管密度(MVD).结果 Endoglin、VEGF、KDR和MMP-9在NCE、CIN及ICC组的阳性率分别为6.7%、38.9%及64.0%;26.7%、61.1%及77.3%;13.3%、61.1%及69.3%和20.0%、55.6%及81.3%,从NCE到CIN再到ICC组,Endoglin、VEGF、KDR及MMP-9的阳性率均显著升高(P<0.05).宫颈癌组织MVD均分别与Endoglin(r=0.499,P<0.01)、VEGF(r=0.602,P<0.01)、KDR(r=0.331,P<0.01)及MMP-9(r=0.287,P<0.05)表达显著正相关,并且Endogin、VEGF、KDR及MMP-9同时均阳性表达者,其MVD升高更为显著(P<0.01).结论 Endoglin、VEGF、KDR及MMP-9联合表达在上调宫颈癌局部肿瘤血管生成中起重要作用,4个指标均过度表达,血管生成能力显著增强.