肺癌患者外周血CD4+CD25+调节性T细胞的检测及意义

摘要

Objective To evaluate the proportion of CD4+CD25+regulatory T cells(Treg)in peripheral blood of patients with lung cancer and observe the relationship of clinical stage,pathologic types,relation between CD4+CD25+ Treg and lung cancer.Methods Flow cytometer was used to analyze the level of CD4+CD25+ Treg in peripheral blood from seventy-eight cases of lung cancer patients and 30 cases of healthy donors.Results The proportion of CD4+CD25+ Treg in peripheral blood was significantly higher in lung cancer patients(21.07±5.26)%when compared with healthy donors(10.04±2.75)%(P＜0.05).The proportion of CD4+CD25+ Treg was significantly higher in stage Ⅲ or Ⅳ lung cancer patients(22.46±4.35)%,(24.35±6.20)% than in stage Ⅰ and Ⅱ lung cancer patients(12.89±3.19)% and healthy donors(P＜0.05).The proportion of CD4+CD25+ Treg was significantly higher in squamous cell carcinoma(20.75±4.85)%,adenocarcinoma(21.52±5.26)% and small cell lung cancer patients(19.20±6.41)% when compared with healthy donors(P＜0.05)but without significance among each teams(P＞0.05).The proportion of CD4+CD25+ Treg was significantly higher in high differentiation (20.36±3.12)%,middle differentiation(21.11±4.67)% and low differentiation(21.30±5.75)% lung cancer patients than in healthy donors(P＜0.05)but without significance among each teams(P＞0.05).Conclusion The proportion of CD4+CD25+ Treg in the peripheral blood of patients with lung cancer is significantly higher than healthy donors.It plays an importmant role in the progression of lung cancer.The up-regulation of CD4+CD25+Treg is not related with pathologic types.%目的 研究肺癌患者外周血CD4+CD25+调节性T细胞(Treg)的比例变化,探讨其与肺癌的临床分期、病理类型及组织学分化程度的相关性.方法 采用流式细胞术检测78例肺癌患者(肺癌组)和30例健康对照者(健康对照组)外周血CD4+CD25+Treg占CD4+T细胞的比例.结果 肺癌组外周血CD4+CD25+Treg占CD4+T细胞的比例为(21.07±5.26)%,与健康对照组(10.04±2.75)%比较,差异有统计学意义(P＜0.05);Ⅲ、Ⅳ期肺癌患者外周血CD4+CD25+Treg比例分别为(22.46±4.35)%、(24.35±6.20)%,显著高于Ⅰ期+Ⅱ期肺癌患者(12.89±3.19)%及健康对照组(10.04±2.75)%相比,差异有统计学意义(P＜0.05);鳞癌、腺癌、小细胞肺癌患者外周血CD4+CD25+Treg比例分别为(20.75±4.85)%、(21.52±5.26)%、(19.20±6.41)%,与健康对照组比较,差异均有统计学意义(P＜0.05),但不同病理类型的肺癌患者间外周血CD4+CD25+Treg比较,差异无统计学意义(P＞0.05);高分化、中分化、低分化肺癌患者外周血CD4+CD25+Treg比例分别为(20.36±3.12)%、(21.11±4.67)%、(21.30±5.75)%,显著高于对照组(P＜0.05),但不同分化程度的各肺癌者间比较,差异无统计学意义(P＞0.05).结论 肺癌患者外周血CD4+CD25+Treg水平明显升高,且与肺癌的进展密切相关,但与病理类型、组织学分化程度无关.