盐酸氨基葡萄糖对兔膝骨关节炎模型关节液中白细胞介素1和白细胞介素6及肿瘤坏死因子α水平的影响

摘要

Objective To explore the effect of glucosamine hydrochloride on interleukin 1 (IL-1),IL-6 and tumor necrosis factor α(TNF-α) in synovial fluid of knee osteoarthritis in rabbits.Methods Twenty adult male rabbits were set up as right knee osteoarthritis models and divided into observation group(n =10) and control group(n =10).The control group was regularly fed;the observation group was added glucosamine hydrochloride 80 mg/kg for 2 months.Bilateral knee joints were scanned by X-ray;IL-6,IL-1 and TNF-α levels in synovial fluid of right knee joint were detected.Results Rats in observation group showed less visible swelling and inflammatory lesion of right knee joint in X-ray images compared to those in control group.Synovial fluid IL-6,IL-1 and TNF-α levels had no significant differences between groups before drug treatment (P ＞ 0.05);the levels in observation group after drug treatment were significantly lower than those before drug treatment and those in control group [(1.7 ± 0.8)mg/Lvs (2.8±0.8),(3.7 ±0.8)mg/L;(0.82 ± 0.27) mg/L vs (1.32 ± 0.43),(1.98 ± 0.46) mg/L;(2.06 ± 0.24) mg/L vs (3.08 ±1.17),(4.13 ±1.25)mg/L] (P＜0.05).Conclusion Glucosamine hydrochloride can reduce IL-6,IL-1 and TNF-α levels in synovial fluid in knee osteoarthritis rabit,thus to alleviate bone lesion and joint injury.%目的 探究盐酸氨基葡萄糖对膝骨关节炎兔关节液中白细胞介素1(IL-1)、IL-6及肿瘤坏死因子α(TNF-α)水平的影响.方法 选择成年雄性兔20只,随机分为观察组和对照组,各10只.2组均制备右膝骨关节炎模型.对照组常规饮食,观察组于造模成功后在常规饮食的基础上喂食盐酸氨基葡萄糖80 mg/kg.给药喂养2个月后对比分析2组双侧膝关节X线影像及右膝关节液中IL-1、IL-6、TNF-α水平.结果 给药2个月后X线片显示观察组右膝关节肿胀不明显,而对照组关节肿胀明显.2组给药前右膝关节液中IL-1、IL-6、TNF-α水平比较差异无统计学意义(均P＞0.05);给药2个月后,观察组IL-1、IL-6、TNF-α较给药前明显下降且明显低于同期对照组[(1.7±0.8)mg/L比(2.8±0.8)、(3.7 ±0.8)mg/L,(0.82±0.27) mg/L比(1.32 ±0.43)、(1.98 ±0.46)mg/L,(2.06 ±0.24) mg/L比(3.08±1.17)、(4.13±1.25) mg/L](均P＜0.05).结论 盐酸氨基葡萄糖可降低膝骨关节炎模型兔关节液中IL-1、IL-6及TNF-α水平,进而缓解骨性病变与关节损伤.