外周血单个核细胞中核因子κB与炎症介质检测对严重多发伤患者的价值

摘要

目的：探讨外周血单个核细胞中核因子资B (NF-资B)与炎症介质在严重多发伤患者中的检测价值。方法选取四川省人民医院(以下简称“我院”)2010年1月~2014年12月收治的严重多发伤患者100例作为观察组,另选取我院同期收治的轻伤患者100例作为轻伤对照组及健康体检者30例作为健康对照组。通过ELISA法原双抗体夹心法测定患者入院第1、2、3、4、7天的外周血淋巴细胞NF-资B及肿瘤坏死因子-α(TNF-α),白细胞介素-1(IL-1),白细胞介素6-(IL-6)的表达,分析并比较三组患者的检测结果。结果观察组和轻伤对照组患者血清TNF-α、IL-1、IL-6、NF-资B水平在入院第1~3天均显著高于健康对照组(P<0.05),并随着时间推移逐渐降低,在入院第4、7天与健康对照组比较差异无统计学意义(P>0.05),趋于正常水平。观察组患者血清TNF-a、IL-6、NF-资B水平在入院第1~3天均显著高于轻伤对照组,差异有统计学意义(P<0.05),第4、7天稍高于轻伤对照组,但差异无统计学意义(P>0.05)。结论严重多发伤患者外周血清单个核细胞中NF-资B及血清前炎症介质细胞因子均成一过性升高,NF-资B及血清前炎症介质细胞因子检测对伤后多器官功能障碍综合征及死亡结局的判定具有参考价值。%Objective To discuss the detection value of peripheral blood neutrophils nuclear factor κB (NK-κB) and inflammatory mediators in patients with severe multiple trauma. Methods From January 2010 to December 2014, in the People's Hospital of Sichuan Province, 100 patients with severe multiple trauma were selected as observation group, at the same period, 100 patients with minor injuries were selected as minor injurys group, and 30 healthy physical exami-nation people were selected as control group. The peripheral blood lymphocyte NF-κB and TNF-α, IL-1, IL–6 of pa-tients treating 1, 2, 3, 4 days were determinate by ELISA -double clamp method, and the determination results of pa-tients in three groups were analyzed and compared. Results Serum TNF-α, IL-1, IL-6, NF-κB of patients in observa-tion group and minor injuries group at admission 1-3 days were statistically higher than those in control group (P <0.05), and gradually reduced with time; at admission 4, 7 days, compared with control group, the differences were not statistically significant (P> 0.05), they returnd to normal levels. Serum TNF-a, IL-1, IL-6, NF-κB of patients in ob-servation group at admission 1-3 days were were statistically higher than those in minor injuries group, the differences were statistically significant (P<0.05), at admission 4, 7 days, compared with minor injuries group the differences were not statistically significant (P> 0.05). Conclusion Peripheral blood NF-κB and serum inflammatory cytokines of pa-tients with severe multiple trauma show a transient rise, Detection value of peripheral blood NF-κB and inflammatory mediators cytokines have some reference value for judging MODS and death.