目的 检测并分析去氧穿心莲内酯的油水分配系数和大鼠肠道吸收特性.方法 采用摇瓶法和大鼠在体肠灌流模型,运用UPLC法测定去氧穿心莲内酯的油水分配系数、化学生物稳定性及其在肠灌流液和胆汁中的含量.结果 去氧穿心莲内酯在pH=6.5的缓冲液的油水分配系数Log P=1.45±0.12,其化学和生物稳定性均良好;在十二指肠、空肠、回肠和结肠的吸收率分别为30.29％、28.39％、22.04％和34.63％;去氧穿心莲内酯胆汁排泄量为灌流药物总量的0.146％.结论 去氧穿心莲内酯在大鼠的各肠段均有吸收,不同肠段吸收率无显著性差异.%Objective To measure and analyze the oil-water partition coefficients and intestinal absorption characteristics of deoxyandrographolide in rats.Methods The shaking method and rat intestinal perfusion model in vivo was used.Oil-water partition coefficient,chemical and biological stability were measured by UPLC,and contents in intestinal perfusate and bile were determined.Results Oil water partition coefficient Log P=1.45±0.12 of deoxyandrographolide in the HBSS (pH 6.5),and its chemical and biological stability were well.In the perfusion model,the absorption of deoxyandrographolide in duodenum,jejunum,ileum and colon were 30.29％,28.39％,22.04％ and 34.63％.On the other hand,deoxyandrographolide was excreted by bile with 0.146％ of total prefused amount of deoxyandrographolide.Conclusion Deoxyandrographolide in various intestinal segments of rats are absorbed,but there is no significant difference on absorption rate in different intestinal segments.
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