乳腺癌患者PTEN、p-AKT蛋白表达情况变化及相关性

摘要

Objective To investigate the expression and significance of protein tyrosinase gene (PTEN) and phosphorylated protein kinase (p-AKT) in patients with breast cancer.Methods 91 cases of breast cancer tissues in West China Hospital from February 2014 to July 2016 were selected,and normal breast tissues from the edge of tumor tissues 1 cm were selected as control group,immunohistochemical method was used to detect the expression of PTEN and p-AKT protein.Results The positive rate of PTEN expression in breast cancer tissues was significantly lower than that in adjacent tissues (P ＜ 0.05),but the p-AKT protein expression was higher than the adjacent tissue (P ＜ 0.05).The PTEN positive expression rate in patients with axillary lymph node metastasis was significantly lower than that in patients without axillary lymph node metastasis (P ＜ 0.05),while the positive expression of p-AKT protein was significantly higher than that without axillary lymph node metastases (P ＜ 0.05).The expression of PTEN protein and p-AKT protein was related to the histological grade (P ＜ 0.05),the expression of p-AKT protein was related to TNM stage (P ＜ 0.05),the expression of PTEN protein and p-AKT protein were not correlated with age,menopausal status,tumor size,estrogen receptor and progesterone receptor in patients with breast cancer (P ＞ 0.05).There was a negative correlation between the expression of PTEN protein and p-AKT protein in breast cancer (r=-0.337,P ＜ 0.05).Conclusion The expression of PTEN protein is decreased in breast cancer,the expression of p-AKT protein is increased,and the expression of PTEN protein and p-AKT protein are negatively correlated,which may play an important role in the development of breast cancer.%目的 探讨乳腺癌患者蛋白酪氨酸酶基因(PTEN)、磷酸化蛋白激酶(p-AKT)蛋白表达及意义.方法 选取2014年2月～2016年7月华西医院收集的乳腺癌组织标本91例,同时选取距肿瘤边缘大于1 cm正常乳腺组织作为对照,采用免疫组化法检测PTEN和p-AKT蛋白表达.结果 乳腺癌组织PTEN表达阳性率明显低于癌旁组织(P＜0.05),而p-AKT蛋白表达阳性明显高于癌旁组织(P＜0.05);腋窝淋巴结转移患者PTEN蛋白表达阳性率明显低于无腋窝淋巴结转移患者(P＜0.05),而p-AKT蛋白表达阳性明显高于无腋窝淋巴结转移患者(P＜0.05).PTEN蛋白和p-AKT蛋白表达与患者组织分级有关(P＜ 0.05);p-AKT蛋白表达与患者TNM分期有关(P＜0.05);PTEN蛋白和p-AKT蛋白表达与乳腺癌患者年龄、绝经情况、肿瘤大小、雌激素受体和孕激素受体无相关性(P＞0.05);乳腺癌患者PTEN蛋白和p-AKT蛋白表达呈负相关性(r=-0.337,P＜0.05).结论 乳腺癌组织PTEN蛋白表达降低,p-AKT蛋白表达升高,且PTEN蛋白和p-AKT蛋白表达呈负相关,可能在乳腺癌发生、发展中有重要作用.