新西兰大白兔骨膜下组织工程骨异位成骨的实验研究

摘要

目的:利用新西兰大白兔骨髓基质干细胞构建组织工程骨,研究其在股骨骨膜下异位成骨的可行性.方法:选取3月龄的清洁级雌性新西兰大白兔,体重3 kg,取其骨髓基质干细胞诱导为成骨干细胞,扩增后接种到β-磷酸三钙生物陶瓷颗粒中,构建的组织工程骨种植到股骨骨膜下,3个月后实验动物血管内灌注凝胶墨汁,通过光学显微镜直接检测组织工程骨的血供和成骨结果.结果:16个标本中的12个植入的组织工程骨颗粒均良好固定在骨膜下并被骨膜包围,组织工程骨中有大量血管和新生骨形成,骨组织结构相对紊乱,不同于正常骨组织结构排列规则,血管分布均匀.4例取材时发现植入物游离于骨膜外,大部分材料被吸收,残留植入物体积明显小于骨膜下成骨,未见明显的骨组织形成,血供情况欠佳.股骨骨膜下组织工程骨颗粒80%贴附牢固,成骨良好,新骨内有大量血管长入.结论:组织工程骨可以在骨膜下获得良好的异位成骨.%Objective: To study the feasibility of ectopic bone formation for engineered bone constructs with bone marrow stromal cells (BMSCs). Methods:BMSCs were obtained from 3-month female New-Zealand rabbits with weight of 3 kg,induced to osteogenitor cell, were expanded by culture and then seeded into the porous p-tricalcium phosphate ((3-TCP) particles. Engineered bone constructs were implanted under the periostem of rabbit femur. Samples were retrieved and examed after 3 months. Blood vessels and osteoblast were examined through optical microscope. Results: Twelve implanted engineered bone particles of 16 samples were fixed well under periosteum and rounded by periosteum. There were a lot of vessels and new bone in engineered bone. The structure of bone was disorder;the vessels arranged equally. Four cases found implanted bone freed outside of periosteum, lots of implanted material were absorbed, the volume of residual was less than osteogenesis, and lack of blood vessel. 80% engineered bone constructs attached to the femur under the periostem very well, osteogenesis was fine and vessels were growed into new bone. Conclusion: Engineered bone can obtained good ectopic bone under the periostem.