肺挫伤致肺水肿患者血浆中E-SLT,IL-8,TNF-α和ET-1的变化

摘要

目的:本研究观察胸部外伤所致肺水肿病人血中E-选择素、TNF、白细胞介素-8和内皮素的变化.方法:用固相双夹心酶联免疫吸附法和放免技术共测定了24例胸部外伤病人在治疗前后血中E-选择素、肿瘤坏死因子、白细胞介素-8和内皮素浓度.结果:临床肺水肿病人血中E-选择素、肿瘤坏死因子、白细胞介素-8和内皮素的浓度在发病时明显升高,与正常对照组比较,差异有显著性(P＜0.001).治愈后恢复正常,治疗后各项指标与正常对照组比较差异无显著性(P>0.05),但治疗前后差异有显著性(P＜0.001).结论:E-选择素、肿瘤坏死因子、白细胞介素-8和内皮素在肺水肿发病机制中起着重要,随着这些因子的升高出现肺水肿并逐渐加重,这些因子降低后肺水肿逐步消失.因此对这些因子的检测有助于估计病情和评价疗效.%Objective:To study the effect of ET-1 ,TNF-α, E-SLT and IL-8 in plasma of 24 patients with lung contusion.Methods:Double antibody sandwich quantitative ELISA method was used to measure the concentration of E-SLT, and radioimmunoassay (RIA) were used to measure the concentration of TNF-α ,ET-1,and IL-8 in 24 patients with lung contusion and 10 normal control subjects.Results:Compared with those in normal compared group, the plasma concentration of E-SLT, ET-1, TNF-α and IL-8 in lung contusion increased significantly, there were also significant difference of the concentration of ET-1,TNF-α,E-SLT and IL-8 in 24 patients between pretreatment and post-treatment.Conclusions:ET-1,TNF-α,E-SLT and IL-8 might play an important role in lung contusion caused by lung edema.Assay of ET-1,TNF-α, E-SLT and IL-8 is very useful to observe advance of lung contusion and judge therapy effect.