目的膀胱癌患者外周血中特异性角蛋白上皮CK-20mRNA在诊断中的意义.方法以半巢式RT-PCR检测外周血有核细胞成分中CK-20mRNA.结果91例膀胱癌中检出CK-20mRNA 37例,阳性率41%,其中证实有转移的20例中17例阳性(85%),25例健康对照组均为阴性.外周血细胞CK-20mRNA的阳性率在远处转移的病例中显著升高(P＜0.01).结论检测膀胱癌患者外周血CK-20mRNA对早期发现膀胱癌细胞的血行播散有重要参考价值.%Objective: A sensitive method was attempted to detect the specific epithelial keratin CK-20 mRNA in peripheral venous blood from patients with bladder carcinoma. Methods: Reverse transcription coupled with twostep polymerase chain reaction (Nested RT-PCR) was used to detect CK-20 expression in the peripheral blood. Results: Detection of CK-20 expression was positive in 37 of 91 patients withbladder carcinoma (41%). Among 20patients with distant metastasis, 17 were positive (85%). CK-20 was not detected in the blood samples from 25 normal individuals. The frequency of positive CK-20mRNA expression was signficantly higher in those with distant metastasis. Conclusion: The presence of CK-20mRNA expression in peripheral blood may be used as an early indicator of hematogenous metastasis of bladder carcinoma cells.
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