目的研究针刺肝经腧穴对实验性偏头痛大鼠肝干组织G蛋白亚基(Gsa和Gia)含量的影响.方法通过皮下注射硝酸甘油(10mg/kg)建立实验性偏头痛大鼠模型,将动物随机分成正常对照组、生理盐水组、模型对照组和针刺治疗组,运用免疫印迹法(Western b1ot)检测脑干组织Gia和Gsa的含量.结果皮下注射硝酸甘油4h后脑干组织Gsa蛋白含量明显升高(P＜0.01),Gia蛋白含量明显降低(P＜0.01),Gsa/Gia蛋白比值升高;针刺治疗组与模型组比较,脑干组织中Gsa蛋白含量明显降低(P＜0.01),Gia蛋白含量明显升高(P＜0.01),Gsa/Gia蛋白比值降低.结论偏头痛发作可能与大鼠脑干组织G蛋白信号传导系统功能障碍有关,针刺介导的G蛋白信号通路可能是其防治偏头痛的重要机制之一.%Objective: In order to explore the effect of acupuncture the liver channel of points on the content of G protein alpha subunits in the rat brain stem after nitroglycerin infusion. Methods: The model of migraine rats were reproduced in accordance with Knynihar-tassorelli methods by infusing the NO lonor nitroglycerin [glyceryl trintrate (GTN)] i.h, then the rats were divided randomly into four groups: Normal control group, Saline control group, Model control group and Acupuncture treatment group. The content of Giα and Gsα protein in the rat brain stem was analysed by western blot method. Results: The model control group of the content of Gsα protein in the rat brain stem was significantly raised (P ＜0.01), the Giα protein was significantly reduced (P ＜0.01), and the ration of Gsα/Giα was increased by comparison with the normal control group. The acupuncture group of Gsα protein in the rat brain stem went up apparently than the model group (P ＜0.01), and Giα protein was slightly higher than the model group (P ＜0.01), resulting in reducing of Gsα/Giα rations. Conclusion: Our results provided important information for understanding how G-protein-linked signaling system play an important role in the attacking migraine, and the mechanisms of acupuncture on migraine was possibility that the acupuncture mediated the G-protein-linked signal transduction pathway plays an important function.
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