孕期被动吸烟对新生大鼠肺SP-A和SP-B mRNAs表达影响的研究

摘要

Objective:To study if prenatal exposure to sidestream cigarette smoke influences the expression of suffactant protein A(SP-A)and surfactant protein B(SP-B)mRNAs in the lung of rat pups.Methods:Sprague-Dawley rats on pregnant day 1,8 and 15 were exposed to sidestream cigarette smoke in a whole-body exposure chamber for 2 h each day.The pups were delivered by cesarean section on the 20thday of gestation.Reverse transcription polymerase chain reaction(RT-PCR)was used to evaluate relative amount of SP-A and SP-B mRNAs expression.Results:The relative amount of SP-A and SP-B mRNAs in the lung of rat pups did not show any significant difference between the control and smoke-exposed groups at any exposure point.Conclusions:The results indicate that prenatal exposure to sidestream smoke does not influence the expression of SP-A and SP-B mRNAs.%目的研究孕期被动吸烟对新生大鼠肺表面活性蛋白A、B(SP-A,SP-B)基因表达的影响,探讨产前被动吸烟对新生大鼠肺损伤的机制.方法分别将SD大鼠于妊娠第1、8和15天置于吸烟箱内每天2 h被动吸烟建立孕鼠被动吸烟动物模型,孕鼠每天置于吸烟箱内2 h不吸烟者为对照组,于妊娠第20天剖宫取胎鼠,采用反转录聚合酶链反应(RT-PCR)方法检测新生鼠肺组织SP-A、SP-B mRNAs的表达.结果不同孕期被动吸烟SD大鼠所产新生鼠肺组织SP-A、SP-B mRNAs的表达与对照比较和组间无明显差异(P＞0.05).结论孕期被动吸烟对新生大鼠肺组织SP-A和SP-B mRNAs的表达无明显影响.