程序性细胞死亡4 mRNA在结直肠癌组织中的表达及意义*

摘要

Abastract: Objective To study programmed cell death 4 (PDCD4) mRNA expression in colorectal cancer tissues from laparoscopic radical resection, as well as its relationship with the clinical data of colorectal cancer. Methods Specimens of colorectal cancer tissues and normal tissues adjacent to carcinoma from 54 cases were collected in laparoscopic surgery between 2012 and 2014. mRNA level was detected by RT-PCR in colorectal cancer specimens. The relationship between mRNA level and clinical data was analyzed. Results expression in the colorectal cancer tissues reduced or even was not seen, the rate of lowered expression was 64.8% (35/54) with ∆Ct (4.50 ± 0.60); the normal tissues had high expression of with 吟Ct (3.54±0.52), the difference was statistically significant ( <0.05). The mRNA level in the colorectal cancer tissues was correlated with the differentiation degree, lymph node metastasis and tumor infiltration depth, but not correlated with gender, age or tumor location. mRNA expression was lower in patients with lymph node metastases than those without ymph node metastasis. The lower the degree of differentiation and the deeper infiltration, the lower the mRNA expression. Conclusions mRNA expression lowers in colorectal cancers. mRNA level is not correlated with gender, age or tumor location, but correlated with degree of differentiation, lymph node metastasis and tumor infiltration depth.%目的：研究腹腔镜结直肠癌根治术癌组织中程序性细胞死亡4（PDCD4）mRNA的表达，以及与结直肠癌临床资料的相关性。方法收集2012～2014年腹腔镜手术结直肠癌及癌旁正常组织标本54例，采用逆转录-聚合酶链反应（RT- PCR）检测结直肠标本中PDCD4 mRNA水平；分析mRNA水平与临床资料的相关性。结果①结直肠癌组织中PDCD4表达降低甚至不表达，表达降低率为64.8%（35/54），△Ct值为（4.50±0.60）；正常组织中PDCD4高表达，△Ct值为（3.54±0.52），差异有统计学意义（P<0.05）。②结直肠癌组织中PDCD4 mRNA水平与分化程度、有无淋巴结转移、肿瘤浸润深度有关，与性别、年龄、肿瘤部位无关；分化程度越低，有淋巴结转移，浸润越深，则mRNA表达更低。结论结直肠癌中mRNA表达下降，PDCD4 mRNA水平与性别、年龄、肿瘤部位无关，与分化程度、有无淋巴结转移、肿瘤浸润深度有关。