溃疡性结肠炎患者外周血中IL-32表达水平及其意义

摘要

目的 检测溃疡性结肠炎(UC)患者外周血中白细胞介素32(IL-32)表达水平,分析IL-32表达水平与UC严重程度的关系.方法 入组50例UC患者(UC组,根据病情分为轻、中、重度)及30例健康体检者(对照组),分别收集UC组和对照组患者的外周血,用Trizol提取外周血中淋巴细胞总RNA,实时聚合酶链反应检测IL-32 mRNA表达水平;用酶联免疫吸附法检测外周血中IL-32蛋白表达水平,比较两组外周血中IL-32表达水平,以及轻、中、重度UC患者IL-32表达水平.结果 UC患者外周血中IL-32 mRNA和蛋白表达水平与对照组比较,差异有统计学意义(P<0.05);重度UC患者外周血中IL-32表达水平与轻、中度比较,差异有统计学意义(P<0.05).结论 UC患者外周血中IL-32表达增高,IL-32可能参与UC的发病.%Objective To investigate serum IL-32 level in patients with ulcerative colitis (UC) and its relationship with the severity of UC. Methods Fifty UC patients (16 mild cases, 20 moderate cases and 14 severe cases) and 30 healthy controls were enrolled in the present study. Peripheral blood of the UC patients and the healthy controls was collected respectively. Total RNA in PBMCs was extracted by TRIzol Reagent. IL-32 mRNA expression level was detected by real-time PCR. IL-32 protein expression level in serum was assayed by ELSIA. Difference in IL-32 expression level between the UC and control groups was compared using student's t test. Differences in IL-32 expression level among the mild, moderate and severe UC groups were compared using analysis of variance. Results Serum IL-32 expression level of the UC patients was higher than that of the healthy controls both at RNA and protein levels (P < 0.05). Serum IL-32 expression in the severe UC group was statistically higher than that in the moderate and mild UC groups, respectively (P < 0.05). Conclusions Serum IL-32 expression in UC patients is increased. IL-32 might be involved in the pathogenesis of UC.