结肠镜检查人群肛门HPV感染情况调查及危险因素分析

摘要

目的 旨在研究结肠镜检查人群肛门人类乳头状病毒(HPV)的感染情况,并分析肛门HPV感染的相关危险因素.方法 选取2014年11月-2016年11月新疆医科大学第一附属医院内镜中心行结肠镜检查的273例患者行问卷调查,同时采用肛门刷采集脱落上皮细胞,在显微镜下观察病理改变,HC2法联合基因芯片法行HPV-DNA检测及基因分型.结果 在273例标本中检出HPV-DNA阳性8例,总感染率为2.93%,其中4例为(52+58)型,2例16型,1例35型,1例39型,均属于高危型HPV,显微镜下观察均未见明显的病理改变.年龄、文化程度、消化道症状是影响肛门HPV感染的危险因素(P <0.05).结论 结肠镜检查人群存在着一定的HPV感染率.年龄、文化程度、消化道症状是影响肛门HPV感染的危险因素.%Objective To investigate the infection of Human Papillomavirus (HPV) in human colon by colonoscopy and to analyze the risk factors of anal HPV infection. Methods A total of 273 patients who underwent colonoscopy at the Endoscopic Center of the First Affiliated Hospital of Xinjiang Medical University from November 2014 to November 2016 were investigated by questionaires. The epidermal cells were collected by anal brush and the pathological changes were observed under microscope. HC2 method combined gene chip method was used for detection and genotyping of HPV-DNA. Results Of the 273 samples, 8 were positive for HPV-DNA, the total infection rate was 2.93%. Among them, 4 were type (52+58), 2 were type 16, 1 was type 35 and 1 was type 39, all belonged to high-risk types of HPV. No obvious pathological changes were observed under microscope. Age, educational level and gastrointestinal symptoms were the independent risk factors for anal HPV infection. Conclusions There is a certain HPV infection rate in the people receiving colonoscopy. Age, educational level and gastrointestinal symptoms are the independent risk factors for anal HPV infection.