对称性肢端角化病皮损中PrxI和CLASP1的表达

摘要

Objective:To detect the expression of peroxiredoxin I ( Prx I) and cytoplasmic linker associat￣ed protein 1 ( CLASP1) in the lesions of symmetrical acrokeratoderma ( SAK) . Methods:The level of Prx I and CLASP1 mRNA in lesions and perilesional skin from 9 patients and normal skin from 9 healthy controls was detected by RT-PCR and the level of protein was detected by immunohistochemistry. Results: The ex￣pression level of Prx I and CLASP1 mRNA in lesions was (0.94±0.66) and (0.95±0.76), which was higher than those in perilesional skin (0.51±0.20 and 0.56±0.31) and normal skin (0.45±0.26 and 0.47±0.33). The positive cells of Prx I were predominantly located in the stratum spinosum and basal layers in the skin le￣sions, while the positive cells of CLASP1 were located in the stratum spinosum. The number of positive cells of Prx I and CLASP1 in lesions were more than those in the perilesional and normal skin. Conclusion:Over-ex￣pression of Prx I and CLASP1 may be involved in the hyperproliferation and abnormal differentiation of epider￣mal keratinocytes in the lesions of SAK.%目的：：检测对称性肢端角化病( SAK)皮损中过氧化物还原酶I( Prx I)和胞质链接相关蛋白1( CLASP1)的表达水平。方法：用RT－PCR和免疫组化方法检测9例SAK患者腕部皮损及其周围皮肤和9名正常人腕部皮肤中的PrxI和CLASP1mRNA及蛋白质的表达。结果： SAK皮损中PrxI和CLASP1 mRNA水平为(0.94±0.66)和(0.95±0.76),明显高于皮损周围(0.51±0.20,0.56±0.31)和正常皮肤(0.45±0.26,0.47±0.33)。 SAK皮损中Prx I阳性细胞弥漫分布于表皮基底层和棘层,而CLASP1阳性染色主要位于棘层,二者的阳性染色范围及程度明显高于皮损周围和正常人皮肤。结论： SAK皮损中Prx I和CLASP1表达上调,可能参与表皮角质形成细胞过度增殖和异常分化。