绵头雪莲花提取物对UVA照射后人成纤维细胞增殖的影响

摘要

目的:明确绵头雪莲花提取物对UVA照射下的人成纤维细胞的保护作用.方法:制备绵头雪莲花浸膏,将培养的人成纤维细胞分为正常对照组、UVA照射组(10 J/cm2)及UVA+药物组,CCK8法检测细胞增殖活性,并比较各组吸光度值.结果:UVA+药物组漂浮死细胞(低于5％)少于UVA照射组(约20％～30％);UVA+药物组(200 μg/mL)吸光度值为1.16±0.22,高于UVA照射组的0.5±0.12,差异有统计学意义(P<0.05).结论:绵头雪莲花提取物对UVA照射人成纤维细胞具有一定的保护作用.%Objective:To determine the protective effects of the extract from Saussurea laniceps on the proliferation of human fibroblasts induced by UVA.Methods:The extract from Saussurea laniceps was prepared and the cultured human fibroblasts were divided into normal control group,UVA group and UVA+ Saussurea laniceps group.The cell proliferation activity was detected by CCK8 and the OD of cells was detected.Results:Dead cells in UVA+ Saussurea laniceps group were less than 5％,which was less than that in UVA group (20％～30％).OD in UVA+ Saussurea laniceps group was 1.16±0.22,which was higher than that in UVA group (0.5±0.12),with a significant difference (P<0.05).Conclusion:The extract of Saussurea laniceps has protective effects on human fibroblasts proliferation induced by UVA.