T细胞酶联免疫斑点法对潜伏性结核感染诊断价值分析

摘要

目的 T细胞酶联免疫斑点法对潜伏性结核感染诊断价值进行分析.方法 选取2015年1月~2015年8月,在我院进行潜伏性结核感染检查患者400例,首先给予结核菌素纯蛋白生物(PPD)试验,再给予T细胞酶联免疫斑点法(FSPOT)进行诊断.结果 FSPOT检测阳性比例优越于PPD检测患者,差异存在统计学意义(P<0.05).FSPOT检测在CD4<200 U患者中的阳性率与CD4>200 U的患者差异无统计学意义(P>0.05);PPD试验在CD4<200 U患者中的阳性率低于CD4>200 U患者,差异有统计学意义(P<0.05).结论 FSPOT检测潜伏性结核感染诊断阳性比例增加,受到CD4细胞计数的影响小.%Objective To analyze the diagnostic value of T cel ELISpot (FSPOT) in latent tuberculosis infection.Methods 400 patients who received latent tuberculosis infection(LTBI) examination were selected. They ran the tuberculin purified protein biological(PPD)test, and accepted FSPOT to be diagnosed.Results The detection positive rate of FSPOT was significantly superior to that of PPD(P<0.05). The detection positive rate of FSPOT among patients who had a level of CD4<200 U was no difference than that of patients who had a level of CD4>200 U(P>0.05). The detection positive rate of PPD among patients who had a level of CD4<200 U was far less than that of patients who had a level of CD4>200 U(P<0.05). Conclusion FSPOT detecting LTBI significantly increases the diagnostic positive rate,meanwhile it is less affected by CD4 cel count.