purpose In order to grasp the city medical institutions at all levels in use disinfectants microbial pollution, en-hance the management of the use of disinfectants, reduce the nosocomial infection rate. Methods in the use of field collec-tion and medical institutions use disinfectant, evaluate the contaminated bacteria in the acquisition of disinfectants; The medical and health disinfection standard 2012 edition (volume). Results in use disinfectants, percent of pass is 82.6%per-cent of pass is 92%alcohol disinfectant, plant-based peroxide (79.3%), chlorine disinfectant was 97.6%, the iodine disin-fectant was 98%, aldehydes disinfectants was 76%, phenolic disinfectant is 99%; Total number of colonies, which is 5.5%, mold and yeast detection rate was 1.3%, staphylococcus aureus, 2% detection rate, pseudomonas aeruginosa detection rate was 0.8%, beta hemolytic streptococcus detection rate of 1%. Conclusion of the used disinfectant must strengthen manage-ment, preparing and using correctly, regular sampling inspection, reduce the nosocomial infection.%目的：为了掌握该市各级医疗机构使用中消毒剂微生物污染情况，加强使用中消毒剂的管理，降低院内感染率。方法在使用现场采集各医疗机构使用中消毒剂，对采集的消毒剂进行污染菌检测；《医疗卫生消毒标准2012版(合订本)》。结果使用中消毒剂合格率为82.6%，醇类消毒剂的合格率为92%，过氧化物类为79.3%，含氯消毒剂为97.6%，碘类消毒剂为98%，醛类消毒剂为76.0%，酚类消毒剂为99.0%；菌落总数超标率为5.5%，霉菌和酵母菌检出率为1.3%，金黄色葡萄球菌检出率为2.0%，铜绿假单胞菌检出率为0.8%，乙型溶血性链球菌检出率为1%。结论对使用中的消毒剂要加强管理，正确配制与使用，定期抽样检测，降低院内感染。
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