Isobaric tags for relative and absolute quantitation (iTRAQ) is a new isotope labeling reagent developed in 2004, which combined with multiple reactions monitoring (MRM), has been applied to the identification and quantification and verification of the target proteins in a wild range of biological samples, including bacterial, yeasts, body fluid, microorganism, human tissues, and cell. As a new method of quantitative proteomics so far, the technique of iTRAQ allows for the quantitative analysis of 2-8 samples simultaneously and displays its advantages of high throughput, repeatability and high sensitivity. It also provides a potential tech-nology platform for evaluation the toxicity of nano-sliver biomedical materials.%iTRAQ是2004年开发的一种新的同位素标记试剂，结合MRM技术已经被广泛的应用于细菌、酵母、体液、微生物、人体组织和细胞等多种样本的蛋白质鉴定、定量以及对目的蛋白的验证中。目前，由于iTRAQ能同时对2-8组样品进行标记分析并具有高通量、重复性、敏感性高等特点使其为进行纳米银生物医学材料毒性评估提供了有力的技术平台。
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