植酸酶处理植物性蛋白质原料对吉富罗非鱼生长性能及磷利用率的影响

摘要

为研究植酸酶体外预处理植物性蛋白质饲料原料对吉富罗非鱼生长性能、鱼体成分和磷利用率的影响，本试验选用500尾体重为（12.0±0.3）g的吉富罗非鱼，按试验要求随机分为5组，每组4个重复，每个重复25尾鱼，试验期为60 d。以豆粕、棉籽粕、菜籽粕等主要植物性蛋白质为原料设计配方，Ⅰ、Ⅱ、Ⅲ组分别在投喂植酸酶体外预处理植物性蛋白质饲料的基础上添加0%、0.6%、1.2%的Ca（H2PO4）2（MCP）；Ⅳ组为体内水解对照组、Ⅴ组作为空白对照组投喂未处理植物性蛋白质饲料和1.2%MCP，Ⅳ组在此基础上添加1280 U/kg植酸酶。结果表明：与空白组相比，植酸酶体外预处理植物性蛋白质饲料原料可以使吉富罗非鱼的增重率提高3.6%~9.7%，Ⅰ组粗灰分提高率高达32.8%，钙含量由1.58%提高到2.31%，粪磷降低在50%以上；与体内水解对照组相比，Ⅰ、Ⅱ、Ⅲ组总磷的储积量分别提高22.7%、22.2%、16.1%。由此可见，本试验条件下，水产饲料中添加适量的植酸酶体外预处理植物性蛋白质饲料原料可替代MCP的添加。%This study was conducted to evaluate the effects of phytase in vitro pre-treated plant protein ingredients on growth performance,fish body composition and phosphorus accumulation rate of Yoshitomi tilapia.This project chose 500 Yoshitomi tilapia,which were randomly divided into 5 groups with 4 replicates of 25 each for 60 d.The phytase-pre-treated soybean meal,cotton meal and rapeseed meal as the main protein source were formulated.GroupⅠ,ⅡandⅢwere respectively added 0%,0.6%and 1.2%Ca (H2PO4)2 based on phytase in vitro pretreatment plant protein diet, whereas Ⅳgroup added 1280 U/kg phytase on the basis of Ⅳ and Ⅴ groups feeds was included untreated feed and 1.2% MCP.The results showed that,compared with blank control group,the phytase in vitro pretreatment plant protein feed ingredients could make Yoshitomi tilapia weight gain rate increased 3.6% ~ 9.7%.Crude ash increased rate of group Ⅰwas up to 32.8%.Calcium content increased by 1.58%to 2.31%and fecal phosphorus decreased above 50%.Compared with the con-trol group in vivo hydrolysis,the accumulation amount of total phosphorus of group Ⅰ,Ⅱ,Ⅲ were increased by 22.7%, 22.2%,16.1%.The results showed that under the experimental conditions,phytase in vitro pre-treatment plant protein feed in aquatic feed could substitude for MCP adding.