辽东湾海水中甾体雌激素的检测及生态风险评价

摘要

建立了SPE-丹酰化衍生-UPLC-MS/MS海水中雌激素的高灵敏度分析方法,对辽东湾海水中甾体雌激素的浓度水平及分布进行了调查并对其潜在的生态风险进行了评估.结果表明:辽东湾海水样品中检出雌酮,17β-雌二醇,17α-雌二醇,雌三醇和17α-乙炔基雌二醇的浓度分别为(0.714±0.407),(0.089±0.077),(0.009±0.011),(0.008±0.008),(0.001±0.003)ng/L.雌激素浓度在大辽河入海口区域最高,双台子河入海口次之,大凌河及小凌河入海口区域最低.辽东湾海水中的17β-雌二醇等当量浓度(EEQ-E2β)为0.562±0.327ng/L,导致野生梭鱼雌雄同体发生的概率约为0.83％,不足以解释该海域野生梭鱼雌雄同体的高发生率.为了有效管理该海域的环境安全,有必要对原因物质进行进一步解析.%A highly sensitive SPE-UPLC-MS/MS method was based on dansyl derivatization was developed to investigate the concentrations and distribution of steroidal estrogens in seawater in Liaodong Bay, China, and further assess their ecological risk to wild so-iuy mullet. Estrone, 17β-estradiol, 17α-estradiol, estriol and 17α-ethinylestradiol were detected in seawater samples with the average concentrations of 0.714±0.407, 0.089±0.077, 0.009±0.011, 0.008±0.008 and 0.001±0.003ng/L, respectively. The total concentration of estrogens was higher in the sea area adjacent to Daliao River than adjacent to Shuangtaizi River, and was the lowest adjacent to Dating River and Xiaoling Rivers. The average of 17β-estradiol equivalent concentration (EEQ-E2yβ) in Liaodong Bay was 0.562±0.327ng/L, and the probability of intersex caused by steroidal estrogens was approximately 0.83%, which was much lower than the incidence of intersex in wild so-iuy mullet. Therefore, further identification of causal chemicals is needed for effective environmental safety management of Liaodong Bay.