针对东太湖某水源地原水中嗅味物质2-甲基异莰醇(2-MIB)浓度过高问题，检测并鉴定原水中藻类分布及组成，考察并建立藻类与2-MIB的相关性，并利用16SrDNA基因测序方法对其中两种主要丝状藻类进行鉴定.结果表明：两个原水取水口及附近养殖区中2-MIB与藻密度呈明显的线性相关(R2>0.85)，且颤藻目浮丝藻、泽丝藻以及针杆硅藻等占总藻数量的55%以上.值得注意的是，存在于藻细胞内部的2-MIB比例可达68%，水处理过程中需考虑胞内2-MIB的释放风险.此外，对养殖区周围水质数据分析表明采样点中2-MIB浓度、藻密度、氨氮浓度均和其与离养殖区距离呈负相关性，表明该地区取水口附近的水产养殖可能是导致2-MB偏高的主要原因.%To figure out the reasonof severe odor problem in the water source of East Taihulake, the algae species and cell densities, as well as their correlationswith 2-MIBconcentration were investigated. Two dominated filamentous algae species in the water were identified according to16S rDNAdetection. The result indicated thatthe algae cell density and the concentration of 2-MIB were of high correlation with R2>0.85.Sometypical odor compounds producer Oscillatoriales (includingthe identified species Limnothrix and Planktothrix) and Synedraaccount for over 55% of the total algae cell numbers. In particular, the intracellular 2-MIB account for 68%of its total concentration, indicating the risk of intracellular 2-MIB release during the water treatment. In addition, the density of algae as well as the concentration of 2-MIB and ammonia nitrogen were decreased withthe increasing distance between sampling points and the aquiculture zone, suggesting that the high concentration of 2-MIB around the intakes wasprobably caused by the aquiculture activities.
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