采用固相萃取-高效液相色谱-串联三重四级杆质谱联用法(SPE-HPLC-MS/MS)测定河北潘家口水库中4种氯霉素类抗生素.该方法采用电喷雾电离源、多重反应监测正或(负)离子模式,4种氯霉素类抗生素加标回收率高于90%,相对标准偏差在1.60%~5.43%.方法的检出限在0.06~0.29ng/L,定量限在0.18~0.87ng/L.潘家口水库水样检测氟苯尼考残留量在26.21~233.35ng/L,氟苯尼考胺在0.53~8.18ng/L,所有水样中氯霉素和甲砜霉素均未检出.对潘家口水库氯霉素类抗生素残留的生态风险和人体健康风险评估表明,RQ(风险商)小于1,说明潘家口水体氯霉素类抗生素对浮游生物和人体健康尚不存在明显危害.丰水期成人和儿童的RQ均比平水期大,最大相差2个数量级.这可能由于此时期为网箱养鱼活跃期,或与水库调水有一定相关性.%Four chloramphenicols, including chloramphenicol, thiamphnicol, florfenicol and florfenicol amine, were investigated in the water from the Panjiakou Reservoir located in Hebei Province, by utilization of solid phase extraction-high performance liquid chromatography-tandem spectrometry (SPE-HPLC-MS/MS). Electrospray ionization and multiple reactions monitoring in positive or negative ionization mode were adopted as the target compounds for qualitative and quantitative analysis. The recoveries of target compounds were all above 90%, with the relative standard deviations (RSDs) ranged between 1.60% and 5.43%. The limits of detection (LODs) and limits of quantification (LOQs) for target compounds were in the range of 0.06~0.29ng/L and 0.18~0.87ng/L, respectively. The detected concentrations of florfenicol in the water samples were in the range of 26.21~233.35ng/L, and detected florfenicol amine were in the range 0.53~8.18ng/L. However, chloramphenicol and thiamphnicol were not detected in the water samples. Additionally, the risk assessments showed that the risk quotient (RQ) for detected antibiotics were below 1, which indicated very low risk to plankton and human health. The RQs of adult and child in wet season were over 100times higher than that in regular level, which might be caused by either the cage culture period or potential impact from water diversion in the reservoir.
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