CYCLOSPORINE BINDING PROTEIN AND USE IN AN ASSAY FOR BIOLOGICALLY-ACTIVE CYCLOSPORINE

摘要

A method of quantitatively estimatingbiologically-active cyclosporines, analogues,derivatives and metabolites by a competitive proteinbinding assay using water-soluble binding proteinsisolated from the cycloplasm of human and animal cells.Tracers include radioactive atoms, fluorescentmolecules, enzymes, and chemiluminescent molecules.Solution and solid phase assay methods are disclosed.Also claimed are the water-soluble binding proteins.