研究了气相保藏条件对厌氧氨氧化菌种短期保藏效果的影响,分别在常温(14±0.5)℃和低温(4±0.5)℃条件下,将厌氧氨氧化菌种在气相条件分别为13393mg/m3的NO、1339mg/m3的NO、氮气和空气的环境中密封保存28d,考察了保藏前后菌种的脱氮性能、颜色、沉降性能和生物量的变化,并对常温条件下保藏的菌种做了活性恢复研究,结果发现:在浓度为1339mg/m3的NO中和氮气中保藏的菌种活性都高于相应温度下在空气中保藏的菌种活性,其中常温条件下4种保藏方式活性保留率依次为23.40%,86.22%,61.54%和46.47%,低温条件下4种保藏方式活性保留率分别为36.53%,47.12%,53.53%和42.95%;在无氧环境中保藏能保持菌种的颜色和沉降性能,在高浓度NO中保藏能降低保藏过程中生物量的损失;不同保藏方式下菌种恢复过程中各阶段的分布存在差异,空气中保藏的菌种适应期为6个周期,而在氮气中保藏的菌种其适应期只有2个周期.%The effect of gas phase conditions on the performance of anaerobic ammonium oxidation (ANAMMOX) bacteria under short-term preservation was studied. Under the conditions of normal temperature (14±0.5)℃ and low temperature (4±0.5)℃, ANAMMOX bacteria were preserved in the four gas phase conditions (13393mg/m3 NO, 1339mg/m3 NO, nitrogen and air) for 28days and the recovery experiments of ANAMMOX bacteria preserved under normal temperature condition was studied. The results showed that the activities of the ANAMMOX bacteria preserved in 13393mg/m3 NO and nitrogen conditions were higher than that of the bacteria preserved in the air at the corresponding temperature. The retention rates of ANAMMOX bacteria were 23.40%, 86.22%, 61.54% and 46.47%, respectively,under the four kinds of preservation (normal temperature condition), However, Under low temperature conditions, the retention rates were 36.53%, 47.12%, 53.53% and 42.95%, respectively; The results demonstrated that preservation in oxygen-free environment was beneficial to maintain color and settleability of bacteria while biomass decreased and in high concentrations of NO Can reduce the loss of biomass during preservation. The results of the recovery experiments showed that the distribution of each stage during the recovery process was various in different preservation mode. The adaptation period of bacteria preserved in air was 6cycles while in nitrogen was 2 cycles.
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