Self-powered anti-interference photoelectrochemical immunosensor based on Au/ZIS/CIS heterojunction photocathode with zwitterionic peptide anchoring

摘要

Accurate detection of important biomarkers with ultra-low levels in complex biological matrix is one of the frontier scientific issues because of possible signal interference of potential reductive agents and protein molecules.Herein,a self-powered anti-interference photoelectrochemical(PEC)immunosensor was explored for sensitive and specific detection of model target of cardiac troponinⅠ(cTnI).Specifically,a novel ternary heterojunction served as the photocathode to offer a remarkable current output and a zwitterionic peptide was introduced to build a robust antifouling biointerface.CuInS(CIS)film with porous network nanostructure was first prepared and then modified in order with ZnInS(ZIS)nanocrystals and Au nanoparticles to fabricate the Au/ZIS/CIS heterojunction photocathode.After capture cTnI antibody(Ab)was immobilized,the zwitterionic peptide KAEAKAEAPPPPC was then anchored to compete the immunosensor.The elaborated PEC immunosensor exhibited high sensitivity for target cTnI antigen(Ag)detection,with good anti-interference against reductive agents and nonspecific proteins.This integration strategy of heterojunction photocathode with zwitterionic peptide provides a new sight to develop advanced PEC immunosensors applying in practical biosamples.