Synthesis of a new ratiometric emission Ca^(2+) indicator for in vivo bioimaging

摘要

A novel fluorescent calcium indicator with a 490/582 nm ratiometric emission has been designed and synthesized.The indicator exhibits a highly selective ratiometric emission response to Ca2+ over other metal cations and a large Stokes shift of 202 nm.Moreover,its practical cell imaging capability for intracellular Ca2+ in the resting- and dynamic-state has been demonstrated in human umbilical vein endothelial cells using a confocal laser scanning microscope.