生地提取物对雏鸡免疫器官指数和H9疫苗免疫效果的影响

摘要

This study was aimed to investigate the effect of the Radix rehmanniae extract on im-mune organs indexes and H9 vaccine immune efficacy.1 200 1-day-old healthy chicks fed with bas-al diet were chosen and randomly devided into 6 Groups on 14-day-old:GroupⅠ,Ⅱ and Ⅲ adding 2‰,3‰ 0.4‰Radix rehmanniae extract,respectively;Group Ⅳ adding 1‰ astragalus polysac-charide;Group Ⅴ immunned with H9 vaccine and fed with basal diet;Group Ⅵ was blank control group which didn’t immunned with H9 vaccine and fed basal diet.After 7 days medicine-feeding, the samples were collected at 14,21,28,35,42,49 and 56 days and the immune organs indexes and the H9 antibody levels were determined.The results showed as follows:①Compared with groupⅥ,the spleen and bursa indexes of groupⅠ,Ⅱ,Ⅲ and Ⅳ were significantly increased (P<0.05),indicating that dietary supplementation with Radixrehmanniae extract and astragalus pol-ysaccharide could improve spleen and bursa development (P<0.05).Compared with group ⅠandⅢ,the bursa and spleen indexes of group Ⅱ were significantly increased (P<0.05),while there was no significant difference between group Ⅱ and Ⅳ (P>0.05 ).②Dietary supplementation with Radix rehmanniae extract and astragalus polysaccharide could improve H9 antibody titers and hold it at a higher level.Compared with group Ⅰand Ⅲ,the H9 antibody titer of group Ⅱwas significantly increased from 42 days (P<0.05),while there was no significant difference be-tween group Ⅱ and Ⅳ (P>0.05).In conclusion,the Radix rehmanniae extract could improve immune organs development and immune function of chicks,and keep antibody titers at high lev-els when matches with H9 vaccine.And the recommended supplemental level of Radixrehmanni-ae extract was 2‰ in diet for chicks.%本试验旨在研究生地提取物对雏鸡免疫器官发育和 H9疫苗效果的影响,采用单因素分组设计,选用1200只1日龄雏鸡,随机分为6组,生地提取物添加组(Ⅰ、Ⅱ、Ⅲ组)分别在基础日粮中添加1‰、2‰、3‰生地提取物,黄芪多糖组(Ⅳ组)在基础日粮中添加0.4‰黄芪多糖,免疫不给药组(Ⅴ组)免疫 H9疫苗并饲喂基础日粮,空白组(Ⅵ组)不免疫 H9疫苗并饲喂基础日粮,药物添加期为7 d,分别在雏鸡14、21、28、35、42、49、56日龄进行采样,测定鸡群的 H9抗体水平,计算法氏囊和脾脏的脏器指数。结果显示：①与Ⅵ组相比,Ⅰ、Ⅱ、Ⅲ、Ⅳ组均能显著提高雏鸡脾脏和法氏囊指数(P<0.05),促进雏鸡的脾脏和法氏囊的生长发育,其中,Ⅱ组(2‰生地提取物)的脾脏指数和法氏囊指数较Ⅰ、Ⅲ组显著提高(P<0.05),且Ⅱ组与Ⅳ组无显著差异(P>0.05)。②黄芪多糖和生地提取物的添加可提高雏鸡血清禽流感疫苗抗体效价并且使 H9抗体维持在较高水平(log2),从42日龄起,Ⅱ组的抗体滴度显著高于Ⅰ、Ⅲ组(P<0.05),同时与Ⅳ组无显著差异(P>0.05)。综上所述,生地提取物具有促进雏鸡的脾脏和法氏囊发育的作用,配合 H9疫苗使用,可以维持抗体处于较高滴度,建议生地提取物在雏鸡日粮中的添加剂量以2‰为宜。