采用高速逆流色谱和大孔吸附树脂相结合的技术,对冻青中的黄酮类化合物进行分离纯化.高速逆流色谱溶剂体系为正己烷-乙酸乙酯-甲醇-水(3:4:5；3,体积比),上相为固定相,下相为流动相,螺旋管柱正转800 r·min-1,流动相流速3 mL·min-1,进样量20 mL,进样浓度20 mg·mL-1,检测波长254 nm.分离获得两种高纯度黄酮类化合物,纯度分别为98.01％、96.10％,经HPLC和NMR鉴定为鼠李秦素-3-O-β-D-葡萄糖苷和高圣草素-7-O-β-D-葡萄糖苷.%High-speed counter-current chromatography(HSCCC)combined with macroporous adsorption resins was employed to separate and purify flavones from Viscum coloratum (Kom.)Nakai. The two phase solvent system of HSCCC consisted of n-hexane-ethyl acetate-methanol-water(3:4:5:3,volume ratio)with the stationary phase as its upper layer and the mobile phase as its lower layer,coiled column rotate speed was 800r·min-1 ,flow rate was 3mL·min-1,injection volume was 20mL,sample concentration was 20mg·Ml-1,detection wavelength was 254 nm. Two high-purity compounds, rhamnazin-3-O-β-D-glucoside and homoeriodictyol-7-O-β-D-glucoside were obtained with purity of 98.01% ,96.10% .respectively. And their structures were identified by HPLC and NMR.
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