β-4,4'-二羟基联苯卟啉的合成及其生物活性研究

摘要

β-4,4'-Dihydroxybiphenyl porphyrins were synthesized by reaction of β-porphyrin with 4,4'-di-hydroxybiphenyl directly,and structurally characterized by UV,'HNMR,IR and.MS. Their biological activities were explored also. The results showed that β-4,4'-dihydroxybiphenyl porphyrins possessed remarkable ability to generate single line oxygen and to cleavage pBR322 plasmid DNA under light irradiation. Furthurmore, it maybe bind with CT-DNA in the mode of external self-accumulation.%以β-硝基四苯基金属卟啉为原料,将4,4'-二羟基联苯引入四苯基卟啉的β位得到一系列4,4'-二羟基联苯卟啉,通过紫外光谱、红外光谱、质谱及核磁共振氢谱等对该卟啉化合物进行了结构表征,并考察了其生物活性.结果表明,4,4'-二羟基联苯引入到四苯基卟啉的β位,使其Soret带一定程度上红移；β-4,4'-二羟基联苯卟啉具有明显的产生单线态氧的能力；光照条件下,β-4,4'-二羟基联苯卟啉对pBR322质粒DNA有明显的切割作用；β-4,4'-二羟基联苯卟啉可能是以外部自堆积的方式与CT-DNA发生作用的.