二氢苯骈c菲啶类生物碱的制备及其抗乙肝病毒的活性

摘要

二氢苯骈[c]菲啶类生物碱是自然界中一类重要的生物活性成分,但含量很低.利用硼氢化还原方法,对博落回粗提物中的血根碱和白屈菜红碱混合物进行还原、分离,得到了二氢血根碱和二氢白屈菜红碱；并初步评价了其抗乙肝病毒活性.结果表明,利用硼氢化还原方法制备二氢苯骈[c]菲啶类生物碱的产率较高,还原产物具有一定的抗乙肝病毒活性.%Dihydro-benzo[c]phenanthridine alkaloids are a kind of bioactive constituents, but their contents are very low in natural plants. Thus the mixture of sanguinarine and cheleryth-rine primarily extracted from Macleaya cordata (Willd) R B were reduced by sodium boro-hydride and separated into dihydrosanguinarine and dihydrochelerythrine. The anti-hepatitis B virus (HBV) activity of as-obtained dihydrosanguinarine and dihydrochelerythrine was evaluated. It has been found that phenonthridine alkaloids can be obtained in a high yield via the reduction of sanguinarine and chelerythrine by borohydride. As-obtained dihydrosanguinarine and dihydrochelerythrine products have some inhibitory effect against HBV (HBsAg secretions in Hep G2. 2.15 cell line).