白花丹素镧（Ⅲ）配合物与牛血清白蛋白相互作用的光谱学研究

摘要

白花丹素（Plumbagin ，简称PLN）是从传统中药白花丹中提取出来的具有抗肿瘤活性的萘醌类化合物（2-甲基-5-羟基-1，4萘醌），而白花丹素镧（Ⅲ）配合物[PLN-La（Ⅲ）]对MCF-7、BEL7404和 NIC-460等肿瘤细胞株具有体外细胞毒活性。采用荧光光谱、同步荧光及紫外光谱法研究了PLN和PLN-La（Ⅲ）与牛血清白蛋白（BSA）的相互作用。结果表明，PLN和 PLN-La （Ⅲ）均可通过静态荧光猝灭的方式减弱BSA的荧光强度；同PLN相比，PLN-La （Ⅲ）与BSA的结合常数较小。%Plumbagin (PLN) was isolated from plumbago zeylanica ,an anticancer traditional Chinese medicine .PLN and its complex with La(Ⅲ ) ,La(C11 H7 O3 )3 (H2 O)2 ,were then al-lowed to interact with bovine serum albumin (BSA) .The interactions among PLN and La(C11 H7 O3 )3 (H2 O)2 and BSA were investigated by fluorescence spectrometry ,synchronous fluores-cence spectrometry ,and ultraviolet-visible light absorption spectrometry .It was observed that PLN and La(Ⅲ) complex can reduce the fluorescence intensity of BSA by way of static quench-ing .Besides ,the binding constant of La(Ⅲ) complex with BSA is obviously lower than that of PLN with BSA .