天青A-BSA共振散射光谱研究及分析应用

摘要

考察了天青A与牛血清白蛋白(BSA)混合体系的共振散射光谱,发现天青A的加入能引起BSA的共振散射光谱强度明显增强,通过优化实验条件建立了测定蛋白的新方法. 在pH＝3.78的磷酸氢二钠-柠檬酸缓冲溶液中,固定天青A浓度为1.00×10－5mol/L,当牛血清白蛋白浓度在8.80×10－8～4.40×10－6mol/L范围内与体系的共振光散射强度值呈良好的线性关系,线性方程为IRLS＝69.744c ( mol/L)+59.359,相关系数r＝0.992 8,检出限为3.28×10－8mol/L,方法应用于牛尿样品中白蛋白含量的测定,回收率在97%～106%之间,表明方法具有较高的准确度,结果令人满意.%The resonance light scattering of azure A-BSA was investigated. The intensity of resonance scattering spectrum was obviously enhanced when 1.00×10－5mol/L Azure A was added. A new method of determination of protein was established by optimization of the experimental conditions of resonance scattering spectroscopy of azure A and BSA. In disodium hydrogen phosphate of pH＝3.78, citrate buffer solution resonance light scattering (RLS) intensity showed a good linear relationship in the con-centration range of BSA with 8.80×10－8－4.40×10－6mol/L. The linear equation was IRLS＝69.744 c (mol/L)+59.359, and the detection limit was 3.28×10－8mol/L. Applied to the determination of BSA in bovine urine samples, the recovery was at the range of 97% to 106%, and satisfactory results was obtained.