A novel glucose biosensor has been presented in this paper. First, the TiO2-MWNT-CS composite was immobilized onto the surface of the glass carbon electrode(GCE). then,a stable nano-Pt film was electrodeposited on the TiO2-MWNT-CS modified e-lectrode by applying an constant potential. Finally, glueose oxidase(GOD)and Nafion were modified onto the film subsequently to fabrieate a glueose biosensor. The modified process and the electrochemical characteristics of the resulting biosensor were characterized by cyclic voltammetry( CV) .electrochemical impedance spectroscopy( EIS) ,and chrpnoamperometry. Under the optimal conditions,a linear dependence of the catalytic current upon glucose concentration was obtained in the range of 3. Oxl0-6 tol.45xl0-3 'mol/L with a detection limit of 9. 5xl0-7mol/L(S/iV=3). In addition,the biosensor showed high sensitivity,good stability and excellent selectivity%在玻碳电极表面滴涂二氧化钛纳米颗粒-碳纳米管-壳聚糖(TiO2-MWNT-CS)复合物,待于后在其TiO2 -MWNT-CS修饰电极表面电沉积一层均匀而致密的纳米铂(Pt),然后将葡萄糖氧化酶(GOD)固定于修饰电极表面,最后,为了防止酶的泄漏和干扰物质的干扰,将Nafion滴涂于修饰电极表面,制得新型的葡萄糖传感器.通过循环伏安法,交流阻抗技术和计时电流法考察了电极的电化学特性.在最佳的实验条件下,该传感器在3.0×10-6 ～ 1.45×10-3mol/L范围内对葡萄糖有良好的线性响应,相关系数为0.9967 (n=15),检测下限为9.5×10-7 mol/L( S/N=3).由于纳米粒子间的协同作用,该传感器具有灵敏度高、稳定性和选择性好等优点.
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