培养条件对产朊假丝酵母合成谷胱甘肽的影响

摘要

The effects of culture conditions and medium on production of glutathione by Candida utilis were investigated in shaking-flask culture, including different carbon source, nitrogen source and their concentrations, pH, working volume and inoculum. Glucose and (NH4)2HPO4 were determined with the optimal concentration of 20g/L and 3g/L respectively. The optimal inoculum was 10%. A total glutathione amount of 74mg/L was achieved with the initial glucose concentration of 20g/L. The intracellular GSH content was increased to 1.40% by changing the culture conditions. The effects of the three constituent amino acids of glutathione and ATP on GSH production were also studied.%考察了培养基组成，培养条件对产朊假丝酵母生长和谷胱甘肽合成的影响，包括不同碳源，氮源及其浓度,pH，装液量，接种量等。确定以葡萄糖、磷酸氢二铵作为碳源和氮源，较佳浓度分别为20g/L和3g/L ，接种量为10％ 。在20g/L的糖浓时获得的谷胱甘肽总量可达74mg/L，胞内含量为1.4％左右。此外还研究了三种前体氨基酸和ATP的加入量，加入时间对发酵的影响。