高效液相法测定薏苡中各部位薏苡素的含量

摘要

The determination of Coixol in each part that Coix lacryma-jobi L.var.mayuen Stapf by HPLC.The part of determination are root、stem、leaf and seed.The model of chromatographic column si C18phenomenex250×4.60mm 5 micron in detection,The model of high performance liquid chromatography is LC2000;Mobile phase is acetonitrile of 25%、water of 75% and in the water the phosphate solution of 0.1%,Flow rate is 0.7mL/min;The temperature of the chromatographic column is 30℃,The wave length of detector is 232nm.The consequence of determination show the content of Coixol is root>leaf>seed≈stem.and the content of root is 46mg/100g,the content of leaf is 21mg/100g,the content of seed is 0.8mg/100g,the content of stem is 2mg/100g.so I suggest root and leaf serve as medicine of Coix lacryma-jobi L.var.mayuen Stapf.%使用HPLC法测定薏苡中各部位薏苡素的含量，测定部位分别是根、茎、叶、种。在检测中所用的色谱柱型号是C18phenomenex250×4.60mm 5 micron，高效液相色谱仪型号为LC2000；流动相是乙腈：0.1%磷酸水溶液（25∶75），流速设为0.743/min；色谱柱温度为30℃，检测器波长为232nm。测定结果显示，各部位薏苡素含量从大到小的依次是根>叶>种>茎，其中根的含量为46mg/100g，叶的含量为21mg/100g，种的含量为0.8mg/100g，茎的含量约为2mg/100g。因此，建议多以根、叶作为药用部位。