阿特拉津高效降解菌株的筛选和降解特性研究

摘要

用富集培养法从城市污水处理厂的污泥中分离得到一株能够降解除草剂阿特拉津的降解菌株,命名为L-1.接种于500mg·L-1的阿特拉津无机盐培养基,96h对阿特拉津的降解率达到94.8％,降解效果理想,对其进行菌种的初步鉴定并对其降解特性进行研究.结果表明,该菌株被鉴定为节杆菌( Arthrobacter sp.).通过室内降解效果优化试验,确定菌株L-1对阿特拉津最佳降解条件:温度为30℃,初始pH值范围为7～8.%One atrazine-degrading bacteria named L-l was isolated from sludge of municipal sewage treatment plant by enrichment culture.After 96 hours, L-l could degrade atrazine of 500rag-L"' up to 94.8%.Based on physiological and biochemical testing and 16S rRNA gene sequence analysis, L-lwas identified as Arthrobacter sp.. The optimum degradation conditions were optimized as followinguhe temperature was 301C and the initial pH was 7-8.