建立高效液相色谱-原子荧光光谱法对水产品中4种砷形态[亚砷酸盐As(Ⅲ)、砷酸盐As(Ⅴ)、一甲基砷酸、二甲基砷酸]进行测定.优化后的实验条件:负高压为270 V,灯电流为80 mA,原子化高度为10 mm,硼氢化钾浓度为20 g/L(含5 g/L KOH),载流为体积分数5%的盐酸.方法的线性范围为0~100μg/L,线性相关系数大于0.999.各种砷形态的检出限为0.20~0.45μg/L;测量结果的相对标准偏差为1.98%~4.81%(n=6);加标回收率为88.4%~101.7%.该方法灵敏度高,快速、准确,检测成本低,可用于水产品中砷形态的检测.%A method for determination of four arsenic species[As( Ⅲ ), As( Ⅴ ), MMA, DMA]in aquatic products by high performance liquid chromatography-atomic fluorescence spectrometry was developed. The experiment conditions were optimized. The negative high voltagewhich was 270 V, lamp current was 80 mA, the atomic height was 10 mm, the concentration of potassium borohydride was 20 g/L (with 5 g/L KOH), and carrying current was 5% hydrochloric acid. The linear range of the method was 0-100 μg/L, the correlation coefficients were more than 0.999. The detection limits of various arsenic species were 0.20-0.45 μg/L. The relative standard deviations of detection results were 1.98%-4.81%(n=6), and the addion recovery rates were 88.4%-101.7%. The method is rapid, accurate and sensitive, it is suitable for the determination of arsenic species in aquatic products.
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