淋洗液在线发生离子色谱法测定血液透析水中的F-,NO3-,SO42-

摘要

建立血液透析水中F-,NO3-,SO42-的淋洗液在线发生-离子色谱检测法.采用ICS-1100型离子色谱仪, IonPac AS19型(250 mm×4 mm)色谱柱,淋洗液自动发生装置在线产生高纯度的KOH溶液作为淋洗液,流量为1 mL/min,以电导检测器检测,色谱峰面积外标法定量.3种阴离子的质量浓度与色谱峰面积在测定范围内呈良好的线性关系(r ≥0.9998),检出限为0.002~0.01 mg/L,测定结果的相对标准偏差为0.26%~0.88%(n=6),加标回收率为98.0%~101.1%.该法操作简单,分析快速,灵敏度高,重现性好,能够准确检测血液透析水中的F-,NO3-,SO42-,可用于血液透析水的质量控制.%A method was developed for determining F-,NO3-,SO42-in hemodialysis water by ion chromatogra-phy with on-line eluent generator. The ion chromatograph of ICS-1100 was used with chromatographic column of Ionpac AS19(250 mm×4 mm) and on-line eluent generator which produced high-purity KOH automatically. The flow rate was 1 mL/min and the inorganic anions of F-,NO3-and SO42-were detected by conductivity detector,the external standard method was used for the quantification by peak area of chromatograph. The mass concentration of 3 inorganic anions was linear with peak area of chromatograph in the detection range with the correlation coefficient no less than 0.999 8. The de-tection limits were in the range of 0.002-0.01 mg/L,and the relative standard deviations of detection results were between 0.26% and 0.88%(n=6),and the recoveries were between 98.0% and 101.1%. The method is simple,speedy,sensitive,it is suitable for determination of F-,NO3-,SO42-in hemodialysis water, which can be used for quality control of hemodialysis water.