建立用超高效液相色谱–串联质谱仪对蔬菜中17种氨基甲酸酯类农药残留同时检测的方法.对传统的QuEChERS前处理方法进行优化和改良,将样品置于萃取管中,加入乙腈溶液提取,振荡离心后,将上清液移入净化管中净化,用超高效液相色谱–串联质谱仪进行检测.17种待测农药的质量浓度在5～500 ng/mL范围内与其色谱峰面积线性关系良好,相关系数(r)大于0.99,检出限为0.3～1.6μg/kg.在5,10,50μg/kg 3个浓度水平进行添加回收试验,平均回收率在61.0％～107.6％之间,测定结果的相对标准偏差为1.6％～17.6％(n=6).该方法检测时间较短,检出限低,测量重现性高,适用于蔬菜中17种氨基甲酸酯类农药残留的同时检测.%A method for determination of 17 kinds of carbamate pesticides residue in vegetables by UPLC–MS/MS was established. The traditional pretreatment method of QuEChERS was optimized and improved,in the extraction tube, the sample was extracted by the acetonitrile solution,and after the shock centrifugation,the upper cleaning liquid was transferred into the purifying tube to purify,and then detected by UPLC–MS/MS. The mass concentration of 17 kinds of pesticides had good relationship with its chromatographic peak area between 5–500 ng/mL, and the correlation coefficient (r) was more than 0.99,the detection limits were 0.3–1.6μg/kg. At the level of 5,10,50μg/kg 3 concentration level, the average recovery rate was 61.0％–107.6％,and the relative standard deviation of determination results was 1.6％–17.6％(n=6). This method is suitable for the detection of 17 kinds of carbamate pesticide residues in vegetables with short detection time, low detection limit and high reproducibility.
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