表面活性剂-超声波协同提取荞麦壳黄酮工艺研究

摘要

Surfactant assisted ultrasonic extraction of flavonoids from buckwheat hull was studied. With the yield of flavonoids as index,ultrasonic extraction of flavonoids from buckwheat hull was optimized by the orthogonal experiments. Under the best conditions,the influences of surfactants of SDS,Tween–20 and Tween–80 on the yield rate of flavonoids were illustrated. The results showed that under the best conditions of extraction temperature 80℃,ethanol volume fraction 40%,ultrasonic time 70 min,solid–liquid 1∶50,ultrasonic power 200 W,the yield of flavonoids was 5.176%,add the concentration of SDS 1.5 g/L,the yield of flavonoids was up to 5.445%. surfactant could improve the yield of ultrasonic extraction of flavonoids from buckwheat hull.%　　研究表面活性剂―超声波协同提取荞麦壳黄酮类化合物，以黄酮得率为指标，采用正交实验优化超声波辅助提取荞麦壳黄酮工艺；在此基础上，考察表面活性剂SDS、Tween–20和Tween–80对黄酮得率影响。结果表明，在超声温度80℃、乙醇体积分数40%、超声时间70 min、料液比1∶50、超声功率200 W条件下，黄酮得率为5.176%；添加浓度为1.5 g/L至SDS后，黄酮得率提高至5.445%，表明表面活性剂对超声波提取荞麦壳黄酮具有一定增效作用。