The collagen from cod skin was extracted by hot-water extraction and enzymatic hydrolysate of the hot-water extracted collagen was hydrolyzed with collagenase. The hot-water extraction conditions were optimized with response surface methodology (RSM) to obtain the best extraction conditions as follows:hydrolysis temperature of 80 ℃, extraction time of 9 h, solid-liquid ratio of 1∶17 (mg/mL). The highest extraction yield was predicted to be 41.95% by the RSM model and the average yield of three verified experiments was 41.78%, which was closed to the predicted value. The hot-water extracted collagen from cod skin was enzymatic hydrolyzed by collagenase and the best hydrolysis conditions were optimized with RSM to be enzyme-substrate ration of 50.0 U/mg, substrate concentration of 8.0 mg/mL, hydrolysis pH 5.5, and hydrolysis temperature of 33 ℃. The highest hydrolysis degree was predicted to be 59.55% by the RSM model and the average yield of three verified experiments was 58.32%, which was closed to the predicted value.%以鳕鱼加工副产物鱼皮为原料,优化研究热水提取胶原蛋白的工艺条件.通过响应面得到最佳提取条件为温度80 ℃、时间9 h、料液比1∶17 (mg/mL),响应面模型预测该条件下胶原蛋白得率为41.95%,验证实验得率为41.78%,与预测值接近.以热水提取的鳕鱼皮胶原蛋白为原料,通过响应面优化胶原酶水解胶原蛋白的工艺,得到最佳水解条件为酶底物比50.0 U/mg、底物浓度8.0 mg/mL、pH 5.5、水解温度33 ℃,响应面模型预测该条件下水解度为59.55%,验证实验实际水解度为58.32%,与预测值接近.
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