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中文期刊>细胞研究:英文版
>Abrupt decrease of c-myc expression by antisense transcripts induces terminal differentiation and apoptosis in human promyelocytic leukemia HL-60 cells
Abrupt decrease of c-myc expression by antisense transcripts induces terminal differentiation and apoptosis in human promyelocytic leukemia HL-60 cells
This study was designed using c-myc antisense transcripts to evaluate how alteration of c-myc expression in human myeloid leukemic HL-60 cells could influence the myelomonocytic differentiation and induction of apoptosis. The recombinant plasmid pDACx expressing antisense transcripts to c-myc fragment containing a part of intron 1 and 137 nt exon 2 was constructed. pDACx was transfected into HL-60 cell line by lipofectin reagent.Cytochemical stainings including NBT reduction, peroxidase and α -NAE as well as detection of CD13 and CD33 antigens by flow cytometric analysis indicated occurrence of myelomonocytic differentiation in cells expressing antisense transcripts to c-myc. DNA degradation measured by DNA gel electrophoresis and typical morphological changes observed under electron microscope proved the switchon of apoptosis in terminally differentiating HL-60 cells.
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机译:Induction of Indoleamine 2,3-dioxygenase (IDO) Enzymatic activity Contributes to Interferon-Gamma Induced apoptosis and Death Receptor 5 Expression in Human Non-small Cell Lung Cancer Cells