肿瘤干细胞标志物CD133-2、CD24和CD44S在头颈部鳞状细胞癌组织中的表达及其临床意义

摘要

目的 探讨肿瘤干细胞标志物CD133-2、CD24、CD44S在头颈部鳞状细胞癌(HNSCC)组织中的表达情况及其临床意义.方法 采用免疫组织化学SP法,分别检测83例HNSCC患者癌组织、46例癌旁正常鳞状上皮组织中CD133-2、CD24、CD4s的表达,分析表达情况及与临床病理特征的关系.结果 CD133-2在癌组织、癌旁正常鳞状上皮组织中表达率分别为9.64 ％(8/83) 、21.74 ％(10/46),CD24分别为90.36％(75/83)、45.65％(21/46),差异均有统计学意义(x2值分别为15.040、5.818,均P＜0.05)；CD44S在癌组织、癌旁正常组织中均表达,但其染色评分差异有统计学意义(Z=-4.262,P＜ 0.05).在癌组织中,CD133-2的表达与组织的分化程度呈负相关(x2=7.246,P＜ 0.05),CD24和CD44S的表达均与组织的分化程度呈正相关(x2值分别为9.005、44.765,均P＜0.05),CD44S的表达与T分期呈负相关(x2=4.650,P＜0.05).结论 CD24、CD44S在HNSCC中的表达随着肿瘤细胞的分化程度增高而增高,能否作为HNSCC的肿瘤干细胞标志物尚需进一步研究；CD133-2表达随肿瘤细胞的分化程度增高而呈下调趋势,可以作为肿瘤干细胞的标志物,并可能与肿瘤的进展、临床预后相关.%Objective To explore the expressions and significances of the tumor stem cell markers CD133-2,CD24 and CD44s in head and neck squamous cell carcinoma (HNSCC) tissues and their association with the clinical pathologic characteristics.Methods Expressions of CD133-2,CD24 and CD44s were analyzed by immunohistochemistry (SP) in 83 cases of primary HNSCC tissues and 46 cases of normal epithelia.Clinicopathological indexes were assessed.Results In primary HNSCC tissues and normal epithelia,the expression rates of CD133-2 and CD24 were 9.64 ％ (8/83),21.74 ％ (10/46) and 90.36 ％ (75/83),46.67 ％ (21/46)respectively,which had statistically significances (x2 =15.040,5.818,P ＜ 0.05).CD44s expression was detected in primary HNSCC tissues and normal epithelia,but their staining scores had statistical significance (Z =-4.262,P ＜ 0.05).In primary HNSCC tissues,the expression of CD133-2 had negative correlation with differentiation degree (x2 =7.246,P ＜ 0.05),but CD24 and CD44s had positive correlation with differentiation degree (x2 =9.005,44.765,P ＜ 0.05).In addition,the expression of CD44s in primary HNSCC tissues had negative correlation with T classification (x2 =4.650,P ＜ 0.05).Conclusion The expressions of CD24 and CD44s in primary HNSCC tissues are highly up-regulated with tumor cells differentiation,and further research needs to be performed to discover whether or not CD24 and CD44s could be the markers of tumor stem cells of HNSCC.The expression of CD133-2 in primary HNSCC tissues is highly down-regulated with tumor cell differentiation.As one of the tumor stem cell markers of HNSCC,CD133-2 may play an important role in the development and clinical outcomes of tumor.