杉木炭疽病拮抗菌AM53的发酵条件优化

摘要

It was to optimize the fermentation conditions of Bacillus licheniformis. The Plackett-Burman design was used to optimize the fermentation conditions, and the results showed that culture temperature, the initial pH and the rotating speed had significant influence on the count of the living AM53. After the steepest ascent test and Box-Behnken design, the optimal fermentation conditions of AM53 that we got were:culture temperature was 30.5℃, the initial pH was 8, rotating speed was 185 r/min and inoculation volume was 2%, cultured 24 hours. Under this condition, the average of OD600 was 1.861, which means the experimental values agreed with the predicted values, the predicted model was reliable and available for the optimization of AM53 fermentation conditions.%旨在优化地衣芽孢杆菌（Bacillus licheniformis）AM53的发酵条件。通过Plackett-Burman试验筛选到影响AM53活菌数的重要因素为培养温度、初始pH、摇床转速。经最陡爬坡试验和Box-Behnken试验，获得AM53的最佳发酵条件为培养温度30.5℃，初始pH8，摇床转速185 r/min，接种量为5%，培养24 h。结果显示，在此条件下，OD600平均为1.861，其值与预测值基本相符，说明该模型可信度高，可应用于AM53发酵条件优化。