油菜素内酯基因BAS1根系表达载体的构建及烟草的遗传转化

摘要

In order to allow brassinosteroid geneBAS1overexpress in root, the root expression vector pCAMBIA2301-RB7-rbc with root promoter TobRB7 was constructed.Then inactivated gene BAS1 was integrated into the root overexpression vector pCAMBIA2301-RB7-BAS1-rbc, and they were transformed into tobacco byAgrobactrium tumefaciems infection. With the verification by PCR and RT-PCR, the target gene BAS1 was transformed into tobacco genome successfully, and expressed in root;the expression of brassinosteroid receptor kinase geneBRI1 in roots of transgenic plants was affected.%为使油菜素内酯基因BAS1在根系特异表达。首先构建含有根系启动子TobRB7的根系表达载体pCAMBIA2301-RB7-rbc。再将获得的油菜素内酯失活基因BAS1与其整合，得到BAS1基因的根系特异表达载体pCAMBIA2301-RB7-BAS1-rbc，通过农杆菌侵染转入烟草。经PCR和RT-PCR验证，目的基因BAS1已成功转入烟草，并在根系表达，转基因植株中根系油菜素内酯受体激酶基因BRI1的表达受到影响。