简易的豚鼠皮肤单纯疱疹动物模型的建立及应用

摘要

为建立简易皮肤单纯疱疹动物模型，我们用1型单纯疱疹病毒(HSV-1)经皮下注射感染豚鼠背部,并用病毒分离和PCR方法检测感染局部组织HSV-1增殖动态和DNA。结果发现在感染的第3d出现典型的单纯疱疹病变,至第14d疱疹痊愈;在感染的第2d HSV-1滴度最高，以后逐渐下降，至第10d降至最低水平，2周内均能检出HSV-1 DNA。用此模型研究喷昔洛韦对单纯疱疹的疗效。结果证实PCV能明显缩短疱疹的病程，且对HSV-1增殖有抑制作用。这些结果表明该模型是简易且有效的。%To establish a guinea pig model for herpes simplex,guinea pigs were inoculated subcutaneously on the back with Herpes Simplex Virus type 1(HSV-1).The virus was isolated from the tissue of the inoculated site and PCR was performed to investigate the multiplication of HSV-1.A typical lesion of herpes simplex appeared in the 3rd day after inoculation and healed in the 14th day.The HSV-1 titer reached the maximum in the 2nd day,then declined,and came to the minimum on the 10th day.However HSV-1 DNA could be detected throughout the two weeks.The animal model was used in a study on the therapeutic effect of penciclovir (PCV) on herpes simplex .The date showed that PCV could shorten the course of herpes simplex and inhibit the multiplication of HSV-1.