人精浆中性α-1,4-糖苷酶活性与精子透明质酸酶活性的关系

摘要

目的 探讨人精浆中性α-1,4-糖苷酶(中性α-1,4-G)活性与精子透明质酸酶(HYD)活性(HYD阳性反应率及HYD活性强度)的关系.方法 收集年龄24～40岁不育男性精液38例,正常对照12例,根据精浆中性α-1,4-G活性检测结果分为2组:精浆中性α-1,4-G活性正常组(26例)和精浆中性(α-1,4-G活性异常组(12例).24～40岁正常生育男性精液12例作为对照组.用检测盒测定中性α-1,4-G活性,用改良固定底物膜法分别检测精子顶体内HYD阳性反应率及HYD活性强度.结果 不育各组中的精浆中性α-1,4-G活性和精子HYD活性(HYD阳性反应率、HYD活性强度)均显著低于正常生育组(P<0.01).精浆中性α-1,4-G活性与HYD活性(HYD阳性反应率、HYD活性强度)呈显著正相关(r=0.877,r=0.910;P<0.01).结论 精浆中性α-1,4-G活性对精子HYD活性(HYD阳性反应率、HYD活性强度)有明显影响.%Objective To study the relationship among neutral alpha-1,4 glycosidase( α-1 ,4-G)activity of human seminal plasma and the positive rate and activity intensity of hyaluronidase(HYD).Methods Thirty eight samples of semen were derided into 2 groups according to the result of neutral α-1,4-G activity test.Normal birth masculine seminal fluid matched ages was the control group (12cases).Neutral α-1,4-G activity in semen test kits was used to detect the neutral α-1,4-G activity.The positive rate and activity intensity of HYD in 2 groups were examined by our improved fixed-substrate film method.Results There was significant difference between normal birth group and infertile groups(P ＜0.01 ).The relation between neutral α-1,4-G activity and the positive rate of HYD showed a significant positive correlation ( r =0.877 ,P ＜0.01 ); the relation between neutral α-1 ,4-G activity and the activity intensity of HYD showed a significant positive correlation ( r = 0.910, P ＜ 0.01 ).Conclusion The neutral α-1,4-G activity of human seminal plasma may affect the positive rate and activity intensity of HYD.